Document Type : Original Article
Authors
1
Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
2
Department of Chemistry, College of Science, Jouf University, Sakaka 2014, Saudi Arabia.
3
Polymer Materials Research Department, Advanced Technology and New Materials Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt.
4
Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt.
Abstract
Wound healing is a complex and dynamic procedure which demands a proper climate to reinforce quick remedy. Gentamicin is a broad spectrum amino glycoside antibiotic having a potent bactericidal impact, but its implication is restricted by the minimal bioavailability, shortened half-life, and nephrotoxicity. In this work, a controlled release gentamicin sulfate formulation was constructed employing PGA-HA/PVA wound dressing nanofiber. The loss of UV chromophores in gentamicin structure restrains a straightforward emergence of analytical method. In this context, a validated reversed-phase high-performance liquid chromatography method for the rapid quantification of gentamicin sulfate content in the nanofiber delivery system was developed. The nanofibrous scaffold were constructed from hyaluronic acid, polyvinyl alcohol, and Polygalacturonic acid for accelerating wound healing. The morphology of nanofibers were checked by SEM. The mean diameter of the nanofibers were 360 nm. The FT-IR characterizations disclosed the incorporation of gentamicin into the nanofibers. The presence of gentamicin induces changes in the mechanical properties via influencing the material's ability to withstand tensile forces and deformation. In vivo wound healing on the wistar rats disclosed a faster healing for animals treated with PGA-GM-HA/PVA nanofiber that confirmed by histopathological examination compared to those applied sterile gauze.
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