Artemisia Annua Response to Different Applications of Banana Peel Nanoparticles

Document Type : Original Article


1 Department of Environmental Sciences and Industrial Development. Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt.

2 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt.

3 Department of botany and microbiology, faculty of science, Bani-Suef University, Egypt.


This study was conducted as a randomized experiment to investigate the effects of banana peel nanoparticles on the growth and biovital activity of Artemisia annua . The seeds of Artemisia annua were divided into four groups. Each group was soaked in different solutions: distilled water, nanoparticles dissolved in distilled water, nanoparticles dissolved in a free bacterial medium, and nanoparticles combined with the endophytic Kocuria artinae bacterium dissolved in water. Furthermore, one group was treated with banana peel nanoparticles and the endophytic bacterium grown in a bacterial medium. Each group was further subdivided into three subgroups, which were soaked for 4, 8, and 16 hours, respectively. The prepared banana peel nanoparticles derived from banana peel were characterized using various techniques such as Fourier-transform infrared spectroscopy (FT-IR), potential analysis (ZETA), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray fluorescence spectroscopy (XRF). Plant growth parameters including plant height, plant fresh weight, plant dry weight, and the concentration of artemisinin, as an indicator of plant bioactivity were measured. The results indicated that all growth criteria and parameters exhibited significant differences among the groups. The expression of artemisinin biosynthetic pathway genes, including ADS, FDS, DBR, CYP, CPR, and HMG, was also investigated. The findings demonstrated that the expression of these genes played a role in the biosynthesis of artemisinin through specific pathways, and all genes collectively contributed to its production.


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