Phytochemical Profiling of Hippobroma longiflora Leaf Extract Using LC-MS/MS Analysis and Pharmacological Potential

Document Type : Original Article


1 Department of Biology, Faculty of Mathematics and Natural Sciences, Jalan Agatis-Dramaga Campus, IPB University, Bogor 16680, Indonesia

2 Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

3 College of Pharmaceutical Sciences Makassar, STIFA Makassar, Jalan Perintis Kemerdekaan Km. 13.7 Daya, Makassar 90242, Indonesia


Hippobroma longiflora is a weed plant with diverse metabolites, including alkaloids, phenolics, tannins, and saponins. The community in several tropical regions uses this plant as medicine, but information about the metabolite profile contained in its leaves is still quite limited. Therefore, this research was conducted to identify the metabolite content in Hippobroma longiflora leaf extract and investigate its potential pharmacological effects to ensure its use in healthcare. The method used was LC-MS/MS, followed by inputting the results of LC-MS/MS into MS Dial and MS Finder applications to identify the essential compounds. The analysis resulted in 29 compounds identified: phenolic, terpenoid, and alkaloid compound groups. Hippobroma longiflora contains several potent antioxidants, antibacterial or antiviral compounds, and anticancers. In conclusion, Hippobroma longiflora leaf extract is rich in compounds that can be utilized by the community in medicine and healthcare, in particular for certain infectious diseases and anticancer.