Isolation and Characterization of Some Haloalkaliphilic Fungi from Egypt

Document Type : Original Article


Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo Egypt.


Aspergillus salinarum, Cladosporium sphaerospermum, and Penicillium camemberti were isolated as haloalkaliphiles from Egyptian soils. The three fungal isolates tolerate pH (5 – 11), and they grew on NaCl to 25% (w/v). They were properly recognized morphologically and genetically. Aspergillus salinarum is obligately halophilic fungus required at least 5% (w/v) NaCl and tolerated to 25% (w/v) NaCl at pH 10.0. They prefer KCl on NaCl, the obligate one tolerates 30%(w/v) KCl. Sucrose was preferred on sugar alcohol used followed by glycerol. Increasing glycerol concentration increased the tolerance of these fungal isolates to pH and salt. A screen for the ability to produce extracellular enzymes showed that they produce amylase, protease, cellulase and lipase on different conditions of growth. These fungal isolates have many biotechnological applications.


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