Document Type : Original Article
Authors
1
Biochemistry Department, Research Institute of Ophthalmology, Giza, Egypt.
2
Physics Department, Faculty of Science, Helwan University, Cairo, Egypt.
3
Physiological Optic Unit, Vision Science Department, Research Institute of Ophthalmology, Giza, Egypt.
4
Medical Retina Department, Research Institute of Ophthalmology, Giza, Egypt.
5
Chemistry Department, Biochemistry division, Faculty of Science, El Fayoum University, El Fayoum, Egypt.
Abstract
Diabetes may lead to oxidative stress-related ocular complications including
cataract. Thus, the aid of antioxidant therapy versus oxidative stress development is
necessary to reduce cataract progression. Flavonoid Naringenin is a well- recognized
natural antioxidant. Liposomes are appropriate nano-carriers for ocular drug delivery.
Hence, we aimed to prepare and assess the potential of Naringenin loaded liposome
(NLL) as topical ocular drug to ameliorate streptozotocin (STZ)-induced diabetic
cataract. NLL was formulated by thin lipid film method (0.241 mg/ml); its
characterization was confirmed by Transmission Electron Microscope (TEM)
imaging, zeta potential and Differential Scanning Calorimetry (DSC) measurements.
60 male Wistar rats have been distributed into three groups (control, (STZ) - induced
diabetic cataract and Naringenin treated). NLL was administered as eye drops (in
treated group) twice a day for 12 weeks. Slit-lamp photography and lens inspection
were conducted, FBS was detected. In the lens tissue reduced glutathione (GSH), total
soluble lens protein (TSP), carbonyl protein (CP), interleukin- 6 (IL-6), Caspase-3 and
vascular endothelial growth factor (VEGF) were measured. TEM imaging, particle
size, zeta potential and DSC results exhibited an effective NLL preparation. NLL
exhibited antioxidant, anti-inflammatory and antiapoptotic efficacy in all parameters
associated with oxidative stress in the lens tissue of type 1 diabetic rats. Finally, the
findings disclosed that NLL could be recommended as a potential prophylactic agent
in diabetic cataract.
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