Raman spectroscopy and dynamic light scattering analysis of egg Hen binder behaviors

Document Type : Original Article


1 Egyptian Museum , Ministry of Tourism and Archaeology , Cairo , Egypt

2 Egyptian Petroleum Research Institute (EPRI)

3 National Institute for standards

4 conservation department ,Faculty of Archaeology,Cairo Unversity conservation department ,Faculty of Archaeology,Cairo Unversity


In ancient Egyptian history and the Coptic period, artists used various organic and natural materials to produce specific effects in artifacts as a coating for their paintings. Organic materials play an essential role in the degradation of the archaeological organism, while one of the significant dilemmas is identifying the media themselves. Most of these identification methods are destructive. We used a non-destructive analysis technique, namely infrared Raman spectroscopy, to prevent this drawback. The natural polymeric materials represented by hen egg yolk and white were studied as the widely used material starting from the eighteenth dynasty and in the Coptic period in Egypt regarding the interaction between degraded polymers of hen egg yolk and white and elucidate the mechanisms associated with thermally aging egg white and yolk samples. Raman spectroscopy gave valuable results that well illustrated the behavior of aged samples and the interactions between the media used, in addition to dynamic light scattering analysis, which was used to determine the size of proteins, nucleic acids and compounds, to monitor ligand binding, to understand the size of media particles and to draw the carbon footprint of yolks and whites of hen eggs in the steady state And after obsolescence, as these results helped us to preserve, treat and protect precious artifacts