Amino Acid Combined Chitosan Nanoparticles for Controlled Release of Doxorubicin Hydrochloride

Abd El-Ghaffar, Mahmoud Ahmed; Akl, Magda Abdel-Aziz; Kamel, Amira Mostafa; Hashem, Mona Samir

doi:10.21608/ejchem.2017.745.1021

Amino Acid Combined Chitosan Nanoparticles for Controlled Release of Doxorubicin Hydrochloride

Authors

¹ National Research Centre

² Department of Chemistry, Faculty of Science, Mansoura University

³ Department of Polymers and Pigments, National Research Centre

⁴ Polymers and Pigments Department, National Research Centre

10.21608/ejchem.2017.745.1021

Abstract

Therapy with amino acids (TAAI) has large interest recently for tumor treatment. Complementing to our previous studies in biopolymers and amino acids for medical applications this study was addressed to design a unique therapeutic regime consisting of a cocktail from therapy with amino acids (TAAI) and polymer therapy for cancer disease. This regime is depending on synthesized Doxorubicin loaded chitosan - glutamic acid (Cs-Ga-DOX) nanoparticles via ionic gelation path- way. The encapsulation efficiency was 69%. The average size was 20-37 nm with spherical, homogenous structure, and positive zeta potential.
FTIR of Cs-Ga confirmed the formation of amide linkage at 1644 cm-1. The in -vitro release of DOX was examined for both pH 5.5 and 7.4 to be biphasic with mutual burst release followed by sustained release for 168 h to reach 58% at pH 5.5 and 25% at pH 7.4.
This result suggested that Cs–Ga nanoparticles presented a promising mixture of glutamic amino acid and chitosan as pH-responsive nano-carrier for anticancer drugs.

Keywords