Document Type : Original Article
Authors
1
Chemistry Department, Faculty of Science, Cairo University,16213, Giza, Egypt
2
Petroleum Refining Division, Egyptian Petroleum Research Institute, Egypt
3
chemistry,science,ain shams egyptian petroleum research institute, science, cairo
4
Faculty of Science, Cairo University
5
Chemistry department, Faculty of science, Cairo university.Giza, Egypt
6
Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt.
Abstract
In terms of the environmentally friendly processing of organic waste, biodegradation is one of the most promising waste management techniques to have recently emerged. One of the byproducts generated by the methods used to refine crude oil that poses a significant issue is light slack wax. Our effort was to isolate microorganisms from nearby soil that had been contaminated by light slack wax. Then, these microbes were elevated to test their ability to break down hydrocarbon waste. The most effective isolates were physically and biologically analyzed. The screening approach revealed that strains H4 and H6 exhibited the best biodegradation efficiencies for light slack wax, with rates of 88.23% and 84.76%, respectively after 14 days of treatments. After the treatment with H4 and H6 for 2 weeks, hydrocarbons like; aromatics, naphthene, and iso-alkanes were degraded rather than saturated n-paraffins according to the gas chromatographic analyses of the samples. The bacterial isolates were further described using 16s rRNA analysis and showed that two strains were from the genus Bacillus. These remarkable results are due to the bacteria's ability to produce biosurfactants and enzymes that facilitate the breakdown of hydrocarbon waste. Further research and development in this area may lead to implementing effective bioremediation methods for managing hydrocarbon waste and lowering pollution issues.
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