Method validation for determination of eight elements in ammi-visnaga plant using graphite-atomic absorption spectrometer in Egypt

Document Type : Original Article


1 Quality Control Manager, Pt CELL for Pharmaceutical Industries Co. 10th of Ramadan city 44629, Egypt

2 Department of Chemistry, Zagazig University, Zagazig 44519, Egypt.

3 Department of Chemistry, School of Biotechnology, Badr University in Cairo (BUC), Badr City, Cairo, 11829, Egypt

4 Department of Pharmaceutical Analytical Chemistry, School of Pharmacy, Badr University in Cairo, Badr City, Cairo 11829, Egypt


In this study, we developed an analytical method for quantifying eight elements in ammi visnaga plant. The method was to quantify heavy metals including aluminum (Al), iron (Fe), chromium (Cr), selenium (Se), zinc (Zn), and potassium (K) and trace elements including copper (Cu) and manganese (Mn) using graphite atomic absorption spectroscopy technique (GFAAS). Validation of each method was performed according to ICH Q2 parameters. The methods showed linearity with R2 of the regression equation > 0.999. Limit of detection (LOD) was calculated to be 4.7, 2.7, 0.11, 0.13, 0.022, 0.063, 0.38, and 0.03 ppb for Al, Cr, Cu, Fe, Mn, Se, K, and Zn respectively. While limit of quantification (LOQ) was calculated to be 14, 8.1, 0.34, 0.40, 0.067, 0.19, 1.2, and 0.08 ppb for Al, Cr, Cu, Fe, Mn, Se, K, and Zn respectively. The methods are found to be accurate and precise by calculating the accuracy, repeatability, and intermediate precision measurements with RSD < 2. The methods were found robust by applying small changes in digestion time and different graphite furnaces. In this work, we applied the developed methods to measure the concentration of eight elements in ammi-visnaga plant which is sold in the Egyptian market. 119, 1.79, 0.55, 0.68, 1.81, 0.97, 19.8, and 0.68 ppb were the found concentrations of Al, Cr, Cu, Fe, Mn, Se, K, and Zn in the Egyptian ammi visnaga.


Main Subjects