Fabrication and anticancer evaluation of colchicine bound liposomes in Colon Cancer Cell Line

Shaarawy, Nada Hesham; Shafaa, Medhat Wahba; Hammam, Mahmoud; Hassan, Ahmed; Ibrahim, Ayman

doi:10.21608/ejchem.2023.208921.7934

Fabrication and anticancer evaluation of colchicine bound liposomes in Colon Cancer Cell Line

Document Type : Original Article

Authors

¹ Faculty of science, Helwan university, biophysics department, Helwan, Egypt

² Physics Dept., Medical Biophysics Division Faculty of Science Helwan University

³ Physics, Faculty of Science, Helwan University, Cairo, Egypt

⁴ Faculty of science, Helwan university, Botany and microbiology department, Cairo, Egypt.

⁵ Faculty of science, Helwan universiy biophysics department Cairo, Egypt

10.21608/ejchem.2023.208921.7934

Abstract

Treatment of cancer using nanotechnology is an interdisciplinary area of research in science. We intend to use a tumor targeting technique for colchicine-loaded liposomes. Thereafter the attachment of the drug and liposome was studied by transmission electron microscope (TEM) and the drug was successfully encapsulated into the nanoparticles. The size was (318.1 ± 4.44) nm which determined by dynamic light scattering (DLS). In vitro drug release in phosphate buffer saline (0.1M, pH 7.4) showed sustained drug release. Entrapment efficiency of colchicine-loaded liposomes was 97 ± 0.028%. Colchicine-loaded liposomes had an IC50 value of 16.05 g/ml in cytotoxic study utilizing Caco-2 (colon cancer cell line) treated cells, whereas free colchicine had an IC50 value of 25.30 g/ml. When liposomes were utilized in place of free colchicine, the anticancer activity of the drug was increased.

Keywords