Evaluation of the VITEK®2 Cards for Rapid Direct Identification and Antimicrobial Susceptibility Testing of Human Pathogenic Bacteria

El-Amier, Yasser A.; El-hayyany, Luay Y.; Ahmed, Ibrahim K.; Al-Rubaye, Awf A.; Fahmy, Alaa A.; Khattab, Yasameen Z.; Sultan, Mohammed S.

doi:10.21608/ejchem.2023.214813.8066

Evaluation of the VITEK®2 Cards for Rapid Direct Identification and Antimicrobial Susceptibility Testing of Human Pathogenic Bacteria

Document Type : Original Article

Authors

¹ Botany Department, Faculty of Science, Mansoura University, Egypt

² General Directorate of Health in Anbar, Rutba, Anbar, Ministry of Health, Iraq

³ Pharmacy Department Al-Rasheed University/College, Baghdad, Iraq

⁴ Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt

⁵ Medical Surgical Nursing Department, Faculty of Nursing, Port Said University, Port Said, Egypt

⁶ General Directorate of Health in Diyala, Diyala, Ministry of Health, Iraq

10.21608/ejchem.2023.214813.8066

Abstract

The VITEK®2 system may be used in this investigation to quickly identify bacteria and test them for susceptibility. Our goal was to analyse the extended spectrum isolating isolates problem causing infections in Mansoura Hospital, Egypt, throughout a 2-year period from February 2020 to October 2021. We also wanted to assess the bacterial profile and frequency of antibiotic resistance patterns of pathogens. Significant bacteremia was detected in 533 (2.563%) of the 21095 samples. A total of 533 isolates were examined; 502 (92%) of these were correctly recognized to the species level, while 31 (8%) of the isolates were not. VITEK®2 required 3 hours for direct identification reporting. The broth of the isolates was used to assess susceptibility to 8 antibiotics: ampicillin, cefotaxime, tetracycline, clindamycin, azithromycin, ofloxacin, gentamicin, and penicillin. With reporting times ranging from 2.6 to 16.4 h, the frequencies of susceptibility to the 8 antibiotics ranged from 75.5 to 82%, respectively. This approach can enable same-day reporting in contrast to conventional approaches that need one or two days, allowing for improved patient management. Escherichia coli made up 54 (26.6%) of hospital-acquired isolates and 35 (29.4%) of community-acquired isolates, while Staphylococcus aureus came in second with 33 (11.22%) of hospital-acquired isolates and 20 (17%) of community-acquired isolates, and Staphylococcus epidermidis came in third with 21 (7.14%) of hospital-acquired isolates and 18 (15 The 8 antibiotics studied showed high rates of resistance; 685 (30.6%) and 361 (18%) of gram-negative and gram-positive bacteria, respectively.

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