Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract

Al-Madhagy, Somaia. A.; Mostafa, Nada M.; Youssef, Fadia S.; Awad, Ghada. E. A.; Eldahshan, Omayma A.; Singab, Abdel Nasser B.

doi:10.21608/ejchem.2019.10925.1700

Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract

Document Type : Original Article

Authors

¹ Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University. Cario, Egypt,

² Pharmacognosy Department, Faculty of Pharmacy, Sana'a University. Sana'a, Yemen

³ Chemistry of Natural and Microbial Products Department. Pharmaceutical and Drug industries research Division. National Research Centre. Giza, Egypt

10.21608/ejchem.2019.10925.1700

Abstract

In this study, preliminary phytochemical screening of dried leaves of Coccinia grandis showed presence of triterpenoids, phenolics, flavonoids, saponins and sterols. As well as, five phenolic compounds namely; ferulic acid (1), methyl caffeate (2), ligstroside(3), trans-p- coumaric acid (4) and kaempferol-3-O-β-D-glucoside (5) were isolated from selected fraction of the methanol extract of Coccinia grandis leaves. The structures of the isolated compounds were identified using different NMR spectroscopic techniques (1H, 13C NMR, COSY, HSQC, HMBC) and mass spectrometry. Compounds (1), (2), (4) and (5) were isolated for the first time from Coccinia grandis species while compound (3) was isolated for the first time from Cucurbitaceae family. Moreover, the anti-microbial activity of the selected fraction of the methanol extract of Coccinia grandis leaves was tested against a panel of Gram-positive, Gram-negative bacteria and yeast. A considered activity was recorded against Bacillus cereus (inhibition zone of 20 mm with MIC 1mg/ml).

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