The Effect of Processing Physical Parameters on the Mechanical, Microstructure, and Porosity of Gelatin-HA Scaffolds Produced by Freeze-Drying Technique for Biomedical Applications

Document Type : Original Article

Authors

1 Faculty of Science, Physics Department, Mansoura University, 35516 Mansoura, Egypt.

2 Toxicology Department, emergency hospital, Faculty of Medicine, Mansoura University, 35516 Mansoura, Egypt.

3 Faculty of Science, Physics Department,, Mansoura University, 35516 Mansoura, Egypt.

Abstract

Scaffolds made for bone tissue engineering are the most preferred way of addressing the complex challenges of bone defect healing. Natural polymers are extensively explored in this area because of their features (Biodegradability, biocompatibility, natural abundance, and high processing ability). Hydroxyapatite (HA) has been employed as a restorative material because of its bone compatibility following implantation. However, the brittleness of HA, which limits its usage, is typical of ceramic materials. As a result, a gelatin layer was applied to the surface of the HA scaffold to provide hardness. This study uses the Freeze-Dryer technique to create porous HA scaffolds doped with gelatin. The scaffolds' microstructures, porosity, and mechanical characteristics were studied. XRD and FTIR measurements prove the presence of HA in the prepared gelatin-HA Scaffolds. The mechanical properties of the studied scaffolds have high value after adding chitosan and starch to the prepared gelatin-HA scaffolds. The porosity of the composite scaffolds was enhanced depending on the strategies used to create them and the cross-linked agent added to the manufactured scaffolds. The results confirm the excellent agreement between the porosity and mechanical properties of the scaffolds, in addition to the excellent agreement between gelatin and hydroxyapatite.

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History

  • Receive Date: 27 June 2022
  • Revise Date: 15 September 2022
  • Accept Date: 25 September 2022

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