Green Synthesis of Magnesium Oxide Nanoparticles Using Probiotic Strain Lactobacillus Gasseri and Their Activity against Fungal Strains Isolated From Historical Manuscripts

Document Type : Original Article


1 Conservation Department, Faculty of Archaeology, Cairo University, 12613 Giza, Egypt

2 Department of Manuscripts Conservation, Al-Azhar Al-Sharif Library, Cairo 11511, Egypt

3 Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt

4 Food Toxicology & Contaminants Dept., Food Industry & Nutrition Institute, National Research Centre Dokki, Giza 12622, Egypt

5 Botany and Microbiology Department; Faculty of Science; AL-Azhar University; Nasr City; Cairo-11884; Egypt


Herein, green approach was used to synthesize magnesium oxide nanoparticles (MgO-NPs) by harnessing metabolites of probiotic bacteria Lactobacillus gasseri. The bacterial-mediated green synthesis of MgO-NPs was characterized. Data showed that the color of the cell-free filtrate was changed from colorless to white after mixing with metal precursor and exhibited maximum surface plasmon resonance at 240 nm. Moreover, the green synthesized MgO-NPs have a spherical shape with sizes in the ranges of 1–10 nm and are characterized by crystalline nature. The main components of the prepared sample were Mg and O with weight percentages of 42.3 and 36.92% and atomic percentages of 39.07 and 38.7% respectively. The biosynthesized MgO-NPs showed a high inhibitory effect compared to crude extract of probiotic bacteria stain, L. gasseri against ten fungal strains (Aspergillus ustus AM4, Penicillium citrinum AM5, Penicillium chrysogenum AM11, A. terreus AM12, P. citrinum AM13, P. citrinum AZ8, P. citrinum AZ9, A. niger AZ11, P. chrysogenum AZ12, and P. chrysogenum AZ13) which isolated from deteriorated historical paper in a dose-dependent manner. The MIC values of synthesized MgO-NPs were 50–100 µg mL–1 with a zone of inhibition of 12.3 ± 0.6–16.3 ± 0.6 mm based on fungal strain.