Production and Evaluation of the Immunogenicity of an Enterobacter aerogenes Ghost Vaccine in a Mouse Model

Document Type : Original Article


1 Chemistry department, Faculty of Science, Zagazig University, Cairo, Egypt

2 Chemistry Department, Faculty of Science, Zagazig University, Egypt.

3 Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, Saudi Arabia


Enterobacter aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections.
Multidrug-resistant strains have emerged as a public health threat. In this study, we used a non-expensive method called the
sponge-like reduced protocol (SLRP) to create E. aerogenes bacterial ghosts EAGs (deactivated bacteria) and evaluated their
safety and immunogenicity in mice. The mice were given three doses of EAGs or EAGs with an adjuvant and then challenged
with live E. aerogenes bacteria. The results showed that the EAG-immunized mice had higher levels of specific antibodies IgG,
IgA, and IgM, lower bacterial load in internal organs after challenge, and improved histopathological examination of the liver
and spleen compared to a negative control group. The serum from the EAG-immunized mice also had cross-reactivity with
other Enterobacteriaceae pathogens using western blotting and enzyme-linked immunosorbent assay (ELISA). These findings
suggest that the EAG vaccine may be an effective and safe alternative for the prevention and control of multidrug-resistant
infections caused by E. aerogenes. This study showed that E. aerogenes ghosts could potentially be used as a vaccine.