Amifostine Silica Nanoparticles Characterization and Effect on Neuronal Damage in Cisplatin Treated Rats

Document Type : Original Article


1 National Research Centre

2 Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.

3 Pathology , National Research Centre

4 Department of Chemistry, Faculty of Science, Helwan University

5 Department of Chemistry, Faculty of Science, Helwan Universityelwan University


The main obstacle to cisplatin's efficacy in cancer chemotherapy is neurotoxicity. The objective of this study was to create and characterize amifostine (AMF)-loaded silica nanoparticles (SiNPs@AMF), and investigate the cytoprotective effects of this nano-emulsion model of AMF against cisplatin-induced neurotoxicity in male albino rats. The nano-emulsion was prepared using cetyltrimethyl ammonium bromide (CTAB), castor oil (CAO), tetraethyl orthosilicate (TEOS), and amifostine (AMF) as extra stabilizing agent, surfactant, sources for silica, and a model drug, respectively. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to analyse the hydrodynamic average size and particle shape of the produced nano-emulsion of silica and SiNPs@AMF. It was evident that when compared to silica nano-emulsion, the prepared SiNPs@AMF slightly enhanced the particle size. Biomarkers of oxidative stress including malondialdehyde (MDA), nitric oxide (NO), paraoxonase-1 (PON-1) as well nuclear factor kappa B (NF-κB) were determined. Histopathological examination of brain was also done. Results indicated that MDA , NO and NF-κB levels were increased whereas PON-1 decreased following cisplatin injection.The administration of SiNPs@AMF protected against cisplatin-induced histopathological changes. Treatment with SiNPs@AMF returned these alterations to their original state. Our findings suggest that oxidative stress is involved in the neurotoxic effects of cisplatin and the cytoprotective action of SiNPs@AMF ameliorate the neurotoxic effect induced by cisplatin.