Alkaline pH Caused Complete Viral Inactivation And Reduced Chemical Pollutants In Wastewater

Document Type : Original Article

Authors

1 Water pollution research department, institute of environmental research and climate change, National research center,giza,Egypt

2 Water Pollution Research Department Environment and climate change Research institute , National Research centre, Cairo, Egypt

3 Therapeutic chemistry department, Institute of pharmaceutical and drug industry research, National research center, Giza, Egypt

4 Therapeutic Chemistry Department, National Research Centre (NRC), 33 EL Bohouth St. (former El Tahrir St.),

Abstract

Although different wastewater treatment methods were developed but none of them could guarantee viral removal and inactivation. That made reuse of treated wastewater a cause of viral transmission. Here we evaluated the effect of increasing pH values of wastewater on adenovirus, Herpes simplex virus type 1, rotavirus , coxsackievirus B and Influenza A virus infectivity. On inoculating each virus at a time in distilled water with pH range 8-13, results showed that viral inactivation was directly proportional to increasing both pH and incubation time and that complete inhibition of all tested viruses was achieved upon co-incubation for 1 hour with water at pH12. Same conditions also caused complete inactivation of the same viruses inoculated in sterile-autoclaved waste water. Viral inactivation due to increasing pH value to 12 was also achieved in field samples. Physico-chemical properties of wastewater samples were characterized before and after increasing pH to 12. Results showed that increasing pH caused decrease in heavy metals as well as sulphate, phosphate, bicarbonate concentrations which also suggest pH increase as mean to reduce chemical pollutants of wastewater.

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History

  • Receive Date: 22 November 2022
  • Revise Date: 29 December 2022
  • Accept Date: 15 January 2023

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