Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations

Danial, Semirna; Gamal, Fatma; Saad, Nada; Hamdy, Hala; Adel, Ghada; Elnahas, Mahmoud; Wael, Fady; Aboelghar, Sohair; Hesham, Nada; Tarek, Mohamed; Wagdy, Hebatallah A

doi:10.21608/ejchem.2022.171668.7134

Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations

Document Type : Original Article

Authors

¹ The Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt

² The Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt.

³ Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt

⁴ Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt.

⁵ Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British university in Egypt (BUE), Cairo, Egypt

10.21608/ejchem.2022.171668.7134

Abstract

Abstract

A green simple validated reversed phase ultra -. performance liquid chromatographic method was developed for the analysis of ganciclovir in both bulk and two different dosage.forms. The optimum separation was reached using AcclaimTM RSLC.120 C18…column..(2.1.x.100.mm,.2.2.µm) at 30°C using the mobile phase composed of methanol (60%) and 20 mM phosphate buffer,. pH (4.00) 40%; at 0.5 mL/min. flow rate. The measurement took place at 253 nm using a photodiode array detector. The method was linear at concentration ranged from 20.00 to 300.00 µg/mL. The limit of detection and the limit of quantification were 7.00.µg/mL and 20.00 µg/mL, respectively. The range for the percentage relative standard deviation of intra.-.day was 0.38 to 1.10 %, while for inter-day precisions was 0.56 to 2.08 %, respectively. The method was accurate with percentage recovery ranged from 98.71 to 101.02 % with percentage relative standard…deviation ranged from 0.38 to 1.10 %. The method was favorably applied for determining of ganciclovir in bulk, pharmaceutical tablet and gel preparation. The greenness of the method was assessed by the analytical. eco-scale system and it was found to be eco-friendly.

Keywords