PROFILE COMPOUNDS OF Centella asiatica L. BASED ON GEOGRAPHIC LOCATION AND VARIATION OF SOLVENTS USING FTIR

Burhan, Asril; Syahrir, Muhammad; Aristianti, Aristianti; Rasyid, Andi Ulfah Magefirah; Nurisyah, Nurisyah; Nur, Syamsu

doi:10.21608/ejchem.2022.157641.6829

PROFILE COMPOUNDS OF Centella asiatica L. BASED ON GEOGRAPHIC LOCATION AND VARIATION OF SOLVENTS USING FTIR

Document Type : Original Article

Authors

¹ Sekolah tinggi ilmu farmasi makassar

² Universitas Tompotika (UNTIKA)LUWUK

³ Department of Pharmacology,Hasanuddin University

⁴ Faculty of Medicine and Health Sciences, Muhammadiyah University, Makassar, Indonesia

⁵ Pharmacy department of health polytechnic of the health ministry, Indonesia

⁶ Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi Makassar

10.21608/ejchem.2022.157641.6829

Abstract

Centella asiatica L. is a plant that has long been used as herbal medicine.

The plant's geographical location will affect the levels of bioactive

compounds, which are the general criteria in selecting quality herbal

medicinal raw materials to ensure the consistency of their efficacy. This study

uses FTIR to compare the component profiles of Centella asiatica L. leaves

based on geographic location and solvent variation. Extraction was carried

out by the maceration method using three types of solvents with different

polarity levels, namely ethanol (polar), ethyl acetate (semi-polar), and nhexane (nonpolar). Based on the results of the study, which obtained three

different groups of spot stains from the appearance of TLC, the spectral

results were relatively the same in each variation of the filtrate with different

absorption intensity values, while for FTIR analysis combined with

chemometrics, Bengo and Tombolopao locations had a similarity of 98.95%

were classified in group I, the Cenrana location had a similarity of 93.85%

classified in group II. In contrast, the Bungaya location had 84.85% similarity

in group III. The ethyl acetate extract at the Bengo and Tombolopao locations

had a 99.71% similarity in group I, and the Cenrana location had a 99.17%

similarity in group II. In comparison, the Bungaya location had a similarity of

93.98% in group III, and the n-hexane extract in the Cenrana and

Tombolopao locations had 99 similarities, 84% were classified in group I, the

Bengo location had a similarity of 99.44%, and was classified into group II.

The Bungaya location had a similarity of 97.18% in group III. The research

concludes that there are differences in the profiles of Centella asiatica L. leaf

compounds from the four sampling locations, with variations in the solvents

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