Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum

Document Type : Original Article

Authors

1 Graduate School of Medical Science, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

2 Chemistry Department, Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia

3 Pharmacy College YAMASI, Makassar 90222, Indonesia

4 Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia

5 Department of Oncology, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

Abstract

Red algae are one of the marine creatures which can produce a variety of both primary and secondary compounds. It is widely known that the existence of exploitation will cause species extinction if there is no cultivation. Due to this problem, the use of bacterial symbionts of algae is one of the solutions. This study aimed to obtain bacteria isolates that can produce the L – Asparaginase (L-ASNase) enzyme. The research design was explorative using experimental laboratory methods by using solid substrate fermentation techniques. The sample used was red algae Eucheuma spinosum located on the island of Lae-Lae of Spermonde island of South Sulawesi. Analysis of the data displayed in the form of tables, graphs/images. Based on the results, two species of bacteria-derived L-Asparaginae enzyme. Protein content with the highest of the bacteria Enterobacter genus obtained from fractions 20-40% was equal to 1.780 mg/mL. The highest enzyme activity was in fractions of 20-40% with 32.011 IU/mL. The optimum temperature and pH of each was at 37 °C and pH 7.

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History

  • Receive Date: 22 June 2021
  • Revise Date: 06 July 2021
  • Accept Date: 17 September 2022

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