Synthesis and characterization of lemon essential oil nanoliposomes as potential antimicrobial agents

Document Type : Original Article

Authors

1 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt

2 Biotechnology and Life Sciences Department, Faculty of Postgraduate studies for Advanced Sciences, Beni-Suef University, Egypt.

3 Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Egypt

4 Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt

Abstract

Lemon essential oil (LEO) has antimicrobial efficiency and other biological activities, but its exposure to deterioration from environmental circumstances restricts its application in the food industry. In this work, LEO nanoliposomes were formulated to enhance LEO characteristics, using the modified heating method (Mozafari method). Four different concentrations of LEO were synthesized and characterized. For the first day of preparation, the size ranged from 124.4 to 215.7nm and zeta potential ranged from -69.35 to -80.0 mV, depending on LEO content, and PDI (< 0.6). After 60 days of storage at 4 °C, the results indicated high stability. High-resolution transmission electron microscopy (HRTEM) analysis confirmed the spherical structure of the nanoliposomes. Encapsulation efficiency (EE) was determined by GC–MS. LEO was successfully encapsulated in nanoliposomes with EE (> 96 %). Free LEO and encapsulated LEO were tested as antimicrobial agents in a commercial chicken soup as a food system. The antimicrobial effect was investigated against Staphylococcus aureus and Escherichia coli as food-borne pathogens for 4 days at 20 °C. The results revealed that LEO nanoliposomes have higher activity than free LEO, especially against E. coli. From the previous findings, encapsulated LEO is a promising approach that could be applied in the food industry.

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Egyptian Journal of Chemistry
Volume 65, Issue 132 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part B)
December 2022
Pages 1507-1515

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History

  • Receive Date: 05 July 2022
  • Revise Date: 03 September 2022
  • Accept Date: 07 September 2022

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