Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.

Badawy, Ahmad Muhammad; Zakaria, Abeer Emam; EI-Belely, Ehab Fathy; El-Gamal, Ahmed Darwish; Hammad, Ali Ahmed

doi:10.21608/ejchem.2022.155468.6711

Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.

Document Type : Original Article

Authors

¹ Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority , Cairo, Egypt

² Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority.

³ Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

⁴ Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority

10.21608/ejchem.2022.155468.6711

Abstract

Protein and fatty acid deficiency in human nutrition is a major concern for developing countries. The filamentous blue-green microalga Spirulina platensis is one of the best algal species that can be used as a food supplement. This study aims to optimize biomass, the total protein, and the fatty acid content of Spirulina platensis. Statistics optimization was carried out using the response surface methodology via dry weight and the Kjeldahl method for protein determination, while the fatty acid composition was determined by the conversion of oil to fatty acid methyl esters (FAMEs), which was measured on a gas chromatography (GC) device. The results revealed that the optimum conditions for maximum biomass, total protein, and fatty acid content were in the presence of NaHCO3 at (16.8 g/l) and NaNO3 (2.5 g/l) at temperature (20°C), pH (9), and LI (6000 lux). A prospective study to estimate both essential amino acids and essential fatty acids in S. platensis biomass is recommended.

Keywords