A fast high-performance liquid chromatography method was established to estimate Oxymetazoline hydrochloride and Isoxsuprine hydrochloride in pharmaceutical preparations HPLC. It consists of an Azorbax-C8 (250mm ×4.6 mm, 5µm ) column with a mobile phase composed of methanol:acetonitrile: buffer (0.01 M) potassium dihydrogen phosphate with triethylamine (pH 5.5) (50: 10:40, V/V/V ) eluted at 1.5 ml/min flow rate. The detection was done using a photodiode array (PDA) detector. The proposed method was validated in linearity, precision, and accuracy. The retention times of IsoxsuprineHCl and OxymetazolineHCl are 3.6 and 6.2 ±0.4 min, respectively. The linear range and detection limits were 1-250 µg/ml and 0.5 µg/ml, respectively, for both drugs. The proposed method was successfully applied to determine ISX and OXY in pharmaceutical preparations.
Alnedawi, Z., Hassan, A. M., Hadi, H., & Shabana, A. (2022). Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations. Egyptian Journal of Chemistry, 65(11), 779-784. doi: 10.21608/ejchem.2022.151275.6551
MLA
Zaid Alnedawi; Ali M Hassan; Hind Hadi; Ahmed Shabana. "Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations", Egyptian Journal of Chemistry, 65, 11, 2022, 779-784. doi: 10.21608/ejchem.2022.151275.6551
HARVARD
Alnedawi, Z., Hassan, A. M., Hadi, H., Shabana, A. (2022). 'Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations', Egyptian Journal of Chemistry, 65(11), pp. 779-784. doi: 10.21608/ejchem.2022.151275.6551
VANCOUVER
Alnedawi, Z., Hassan, A. M., Hadi, H., Shabana, A. Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations. Egyptian Journal of Chemistry, 2022; 65(11): 779-784. doi: 10.21608/ejchem.2022.151275.6551
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