Biocompatibility Assessment of Chamomile Volatile Oil, Double Antibiotic Paste in Subcutaneous Tissue of Rats

Document Type : Original Article

Authors

1 Department of Endodontics, Al-Azhar University, Faculty of Dental Medicine for Girls, Cairo, Egypt

2 Medicinal and Aromatic Plants Research Department, National Research Centre, 33 El-Bohouth St., P.O. 12622, Dokki, Giza, Egypt.

3 Endodontic Department, Faculty of Dental Medicine for Girl's Al-Azhar University, Cairo, Egypt.

4 pharmacology, NRC

Abstract

This study aimed to assess the biocompatibility of Matricaria Recutita volatile oil versus 1% double antibiotic paste on subcutaneous tissue in rats through implantation testing. Thirty-six rats were selected for In Vivo assay. The prepared polyethylene tubes were sealed at one end with cyanoacrylate gel carrying the tested medicament and divided into 3 groups (I, II, and III) (36 tubes each) representing the tested material. Group I is the control group (Empty tube), group II comprises 1% DAP (5µl), whereas group III comprises Chamomile volatile oil (2.5µl) according to effective antimicrobial concentration against E. faecalis (ATCC29212), each group was subdivided according to the post-implantation evaluation period at 7, 14, and 21 days. Animals were euthanized and tissue sections were prepared for histopathological and microscopic assessments of inflammation and fibrous capsule thickness & length of each group at different time intervals. The values of fibrous capsule thickness & length (µm) were of the highest value in 1% DAP associated with the highest inflammation intensity followed by the control group, while the lowest value of fibrous capsule thickness & length was in the chamomile group associated with less inflammation, respectively at all-time intervals with statistically significant difference between them. Therefore, chamomile volatile oil exhibits superior biocompatibility over 1% DAP with promising application in endodontics as a biocompatible intracanal disinfectant. 

Keywords

Main Subjects

Egyptian Journal of Chemistry
Volume 65, Issue 132 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part B)
December 2022
Pages 1355-1368

Files

History

  • Receive Date: 21 July 2022
  • Revise Date: 04 August 2022
  • Accept Date: 09 August 2022

Share

How to cite

Statistics