Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils

Document Type : Original Article


1 National Research Centre, 30 El-Tahrir Street

2 1Department of Agricultural Microbiology, Faculty of Agriculture, Benha University, Moshtohor, Kaluybia, 13736, Egypt;

3 Faculty of Agriculture, Benha University, Egypt

4 Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Kaluybia, 13736, Egypt.


Sixteen biosurfactant-producing bacteria were isolated from multiple petroleum-contaminated soils in Kaluybia Governorate, Egypt. Six screening tests (emulsification index (EI24%), oil spreading assay, emulsification activity, cetyltrimethylammonium bromide (CTAB), hemolytic and lipolytic activities) were conducted to select the high producer isolate. Out of 16 isolates, only one was chosen for molecular identification as the most efficient biosurfactant-producer and was found to be Pseudomonas putida ON763757. The type of biosurfactant was determine via three chromatography analytical methods, High-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectrometry analysis (GC-MS). HPLC analysis showed that chlorogenic acid (1409.20 µgg-1) was the main component, followed by ellagic acid (530.12 µgg-1). FTIR and GC-MS analyses indicated that the biosurfactant of Ps. putida was rhamnolipid, which is an exciting product for environmental and industrial applications.


Main Subjects

Volume 65, Issue 13 - Serial Number 13
Special Issue: Chemistry and Global Challenges
December 2022
  • Receive Date: 29 June 2022
  • Revise Date: 17 July 2022
  • Accept Date: 18 July 2022
  • First Publish Date: 18 July 2022