Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation

Mohamed, Sabrein; Issa, Yousry; Al Ansary, Aida L; Mahmoud, Ahmed

doi:10.21608/ejchem.2022.142020.6214

Approach for Quantification of Nanogram Amounts of Hepatitis-C Drugs, Sofosbuvir, and Daclatasvir based on Cerium (IV) Oxidation

Document Type : Original Article

Authors

¹ 1-Department of chemistry, Faculty of science, Cairo University, Giza, Egypt 2-Chemistry Department, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia

² Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

³ Dept. of chemistry, Faculty of Science, Cairo University

⁴ National Center for Environmental and Clinical Toxicology, Cairo University, Giza, Egypt

10.21608/ejchem.2022.142020.6214

Abstract

Sofosbuvir (SOFO) and Daclatasvir (DK) are known as hepatitis-C drugs. Through oxidation of these drugs in a sulfuric acid medium by an excess of Cerium (IV) and the subsequent determination of unreacted Cerium (IV), this research developed spectrophotometric methods for assessing the effectiveness of these hepatitis-C drugs. As an indicator of excessive Cerium (IV), we measured the decrease in the color of four chromotropic acid azo dyes, Chromotrope 2B (method A), Arsenazo I (method B), Spadns (method C), and Sulfonazo III (method D) at 510, 499, 505, and 570 nm, respectively. According to Beer's plots, there was a significant relationship between 0.1-2.1 and 0.1-5.1 µg mL-1 with molar absorptivity values of 1.7x105, 2.7x105, 1.4x105, and 1.7x105 for Sofosbuvir and 1.2x105, 7.0x104, 6.2x104, and 9.0x104 for Daclatasvir utilizing methods Chromotrope 2B (method A), Arsenazo I (method B), Spadns (method C), and Sulfonazo III (method D), respectively. To assess the efficacy of the suggested process, pure and pharmaceutical solutions containing Sofosbuvir and Daclatasvir were tested.

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