Evaluation of vitamin C, proline, enzymes and hydroxymethylfurfural levels in clover honey at different storage conditions

Document Type : Original Article

Authors

1 atfih education adminstration

2 Plant Protection Department, Faculty of Agriculture, Fayoum University

3 Food Science Department, Faculty of Agriculture, Fayoum University

Abstract

Honey is a popular sweetener throughout the world. It is consumed since the most remote times. In this study, the two groups of clover honey (natural honey and adulterated honey produced by the supplementary feeding of bees with sucrose syrup with ratio 2:1) through the nectar flow season were used for study the effect of different storage conditions on honey properties. The obtained honey samples were stored for different periods (3, 6, 9 and 12 months) at room temperature (25º C), refrigerator (-4 ºC) and freezer (-20º C) in the different package (white and black glasses). The levels of vitamin C, proline, enzymes (glucosidase and daistase) and hydroxymethylfurfural in the stored honey were determined. The result noticed that the levels of diastase activity, glucosidase activity and ascorbic acid content were decreased after storage for 12 months at room temperature as compared with those stored in the refrigerator and freezer. Also, the highest losses of diastase activity, glucosidase activity and ascorbic acid content were recorded for honey samples stored in the white glass than those stored in the black glass. While, the proline content of the honey was stable during the first 6 months of storage, then decreased after the next 6 months of storage. On the other hand, levels of hydroxymethylfurfural (HMF) were increased with increasing the storage period at room temperature especially in the white glass. Meanwhile, no significant differences occurred between the levels of HMF in stored honey samples under cooling conditions. It can be concluded that, the honey stored in black glass at low temperatures (refrigerator and freezer) maintained properly the quality of the honey.

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Volume 65, Issue 13 - Serial Number 13
Special Issue: Chemistry and Global Challenges
December 2022
  • Receive Date: 29 March 2022
  • Revise Date: 27 April 2022
  • Accept Date: 04 July 2022
  • First Publish Date: 04 July 2022