Optimization of α-amylase Activity Enzyme Produced By Bacillus subtilis and Aspergillus niger

Document Type : Original Article

Authors

1 Biotechnology and Life Sciences Department, Faculty of Postgraduate studies for Advanced Sciences, Beni-Suef University, Egypt.

2 Microbiology Department, Al -Azhar University, Egypt.

3 Microbiology and Botany Department, Faculty of Science, Beni-Suef University.

Abstract

Many microorganisms are known to be good sources for α-amylase enzyme production, with its recognized industrial importance. Bacillus subtilis and Aspergillus niger are appropriate isolates for production of α-amylase using solid state fermentation (SSF). Efforts were done to increase α-amylase production targeting to decrease the SSF cost. The aim of this study was evaluating certain significant factors as carbon source, temperature, pH, volume of container and minerals for enhancing α-amylase production from B. subtilis and A. niger in lab scale SSF. Results showed that the maximum α-amylase productivity was obtained in units and one unit of enzyme activity defined as the quantity of enzyme that release 1Mmole of sugar/minute, at 37°C for 48 hours incubation or 45°C for 24 hours incubation by used B. subtilis. Inoculum size of 0.5 % v/v was used, and the maximum α-amylase productivity was obtained unit at temperature of 37°C for 7 days by A. niger. The maximum activity was found to be at 60°C using B. subtilis. Inoculum volume of 1000 mL showed the maximum amylase production from B. subtilis, while inoculum volume of 500 mL showed the maximum amylase production from A. niger. ZnSO4 showed the best enzyme production from B. subtilis and FeSO4.7H2O showed the best enzyme production from A. niger. The optimum pH for the best enzyme activity in this study was 6.5 from B. subtilis.

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History

  • Receive Date: 28 May 2022
  • Revise Date: 26 June 2022
  • Accept Date: 29 June 2022

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