Assessment of Cytotoxicity and Genotoxicity Response of Zinc Sulphate on Eukaryotic Cells

Mousa, Shimaa A. A.; Haggran, Abd El-Hamid A.; El-Kawokgy, Tahany M.A; Abo El-Kheir, Zakia A.; Sabry, Shadia M. H.; Rashad, Shimaa E.

doi:10.21608/ejchem.2022.141668.6209

Assessment of Cytotoxicity and Genotoxicity Response of Zinc Sulphate on Eukaryotic Cells

Document Type : Original Article

Authors

¹ Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt.

² Botany and Microbiology Department, Faculty of Science, AL- Azhar University, Cairo, Egypt

10.21608/ejchem.2022.141668.6209

Abstract

A chemical called zinc sulphate (ZnSo4) is inorganic. In order to treat and prevent nutrient deficiencies, zinc is utilised. Zinc is a naturally occurring element that is crucial for tissue development, maintenance, and health. This research used the MTT method to examine the effects of various doses of ZnSo4 on cell viability in hepatocellular carcinoma (HepG2), lung cancer (A549), and normal lung cells (Wi38). Propidium iodide (PI) staining and Annexin V/PI staining were used in flow cytometry to detect both apoptosis and cell cycle arrest appropriately. The current study's findings revealed that ZnSo4 caused cytotoxicity in HepG2, A549, and Wi38 at various doses (IC50 = 308.11, 413.02, and 463.15g/ml). These findings demonstrated that ZnSo4 has cytotoxic effects on both cancerous and non-cancerous cells by reducing cell viability. Byarresting the cell cycle in the G2/M phase and increasing apoptosis, flow cytometry analysis of ZnSo4-damaged HepG2 cells revealed a considerable increase in these two processes. In addition, when HepG2 cell lines were exposed to a high concentration of ZnSo4
, the mRNA expression amounts of p53 and casp3 rose whereas Bcl-2 fell. This study assessed how ZnSo4 affected various yeast haploid knockout strains (YKO). In order to determine the three different ZnSO4 concentrations that this particular set of ZnSO4 could cause DNA damage, we used the comet assay method. The comet assay showed improved yeast cell sensitivity, which has been unquestionably confirmed. The (Clustal Omega Multiple Sequence Alignment EMBL-EBI) alignments of yeast and human gene sequence similarity were used to select the genotypes of YKO.

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