Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model

Hussein, Jihan; El- Bana, Mona; Abdel Latif, Yassmin; El-Sayed, Samah; Youssef, Ahmed; Elnaggar, Mehrez; Medhat, Dalia

doi:10.21608/ejchem.2022.135198.5944

Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model

Document Type : Original Article

Authors

¹ Medical Biochemistry Dept.,National Research Centre,Giza,Egypt.

² Medical Biochemistry Dept., National Research Centre

³ Medical Biochemistry Deart., National Rsearch Centre, Cairo,Egypt.

⁴ Dairy Science Department, National Research Centre, Dokki, Giza

⁵ Packing and Packaging Materials Department, National Research Centre

⁶ Textile Research and Technology Institute, National Research Center

⁷ Medical Biochemistry Dept.,National Research centre

10.21608/ejchem.2022.135198.5944

Abstract

We aimed to evaluate the protective effect of processed cheeses fortified by Laurus nobilis L. extract nanoemulsion against hyperhomocysteinemia in Ehrlich ascites carcinoma (EAC) model. Laurus nobilis L. extract nanoemulsion was prepared via spontaneous emulsification procedure. Then, nanoemulsion was added by different loadings to supplement processed cheeses. The morphology of Laurus nobilis L. extract nanoemulsion was detected using transmission electron microscopy (TEM). Female Swiss albino mice received processed cheese for two weeks then inoculated once with EAC cells. After the end of the experiment, blood samples were collected for determination of serum superoxide dismutase (SOD) , thiobarbituric acid reactive substances (TBARS), B-cell lymphoma 2 (Bcl-2), matrix metallopeptidase-9 (MMP-9), Tumor necrosis factor-α (TNF-α), and homocysteine (Hcy). Results revealed that the formation of Laurus nobilis L. extract nanoemulsion in spherical shape in size around 50 -120 nm. Mice inoculated with EAC cells showed significant increase in serum Hcy, TNF-α, TBARS, and MMP-9 levels while there was a significant decrease in SOD activity and Bcl-2 level. Pretreatment with different concentrations of Laurus nobilis L. extract significantly attenuated oxidative stress, inflammation, and induced apoptosis compared to EAC group.

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