Vitamin B12 is classified as being amongst the hydrophilic vitamins, and plays a crucial role in mortal physiology, and hemoglobin formation and function. It also promotes anti-inflammatory action and moderates risk from viral infection. This paper established an electroanalysis method to quantify vitamin B12 in commercially available supplements by designing a sensor using methylene blue and zinc oxide nanoparticles on a glassy carbon electrode (PMb/ZnO NPs/GCE) via cyclic voltammetry. This offers a wide linear range with high sensitivity for vitamin B12 detection via differential pulse voltammetry using the Co(II/I) redox pair. Sensor morphology and thickness were studied, as was the pH of the supporting electrolyte. To understand the influence co-species, an interference study was also conducted. Under optimized conditions, the redox peak current for Co(II/I) pair at -0.8 V vs. Ag/AgCl, with the linear relationship Ip = 0.0673x + 0.3449, r = 0.9942, showed a linear quantification range for vitamin B12 concentrations of 0.099–69.51 μM. The limit of detection was 0.0104 µM. Repeatability, sensitivity, and stability were evaluated. The PMb/ZnO NPs/GCE electrode developed was successfully applied to the determination of vitamin B12 in commercially available supplements. The recoveries obtained were in the range 97.1-104% for the injection and 95.9-103.3% for the tablet. The results obtained herein compared favorably with those from the current standard quantification via UV–vis spectrometry.
Abd-Alazize, N., & Mohammedtayeb, A. (2023). Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12. Egyptian Journal of Chemistry, 66(2), 375-384. doi: 10.21608/ejchem.2022.135463.5962
MLA
Neam Abd-Alazize; Alaa Mohammedtayeb. "Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12". Egyptian Journal of Chemistry, 66, 2, 2023, 375-384. doi: 10.21608/ejchem.2022.135463.5962
HARVARD
Abd-Alazize, N., Mohammedtayeb, A. (2023). 'Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12', Egyptian Journal of Chemistry, 66(2), pp. 375-384. doi: 10.21608/ejchem.2022.135463.5962
VANCOUVER
Abd-Alazize, N., Mohammedtayeb, A. Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12. Egyptian Journal of Chemistry, 2023; 66(2): 375-384. doi: 10.21608/ejchem.2022.135463.5962
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