Upregulation of Long Non-coding RNA MEG3 and miR-181b in Type 2 Diabetic Patients with Cerebral Stroke

Document Type : Original Article

Authors

1 Therapeutic Chemistry Department, National Research Centre (NRC), El-Bouth St., P.O. 12622 Cairo, Egypt

2 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

3 Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11884, Egypt

Abstract

Background and Aims

Several studies have demonstrated that long non-coding RNA (lncRNA) and microRNA (miRNA) molecules were closely related to the pathogenesis of diabetes mellitus (DM) and its complications. In this study, we aimed to explore the possible relationship of lncRNA MEG3 and miR-181b with the development of ischemic cerebral stroke (CS) as a complication of type 2 DM.

Methods

The study involved 20 diabetic patients (DM), 20 diabetic patients with CS (DM+CS), 17 CS patients and 10 apparently healthy subjects. The expression levels of MEG3 and miR-181b were analyzed in peripheral blood samples using real time PCR.

Results

The expression of MEG3 was significantly higher in both DM+CS and CS groups as compared to DM or control group. In addition, miR-181b was significantly upregulated in DM+CS and CS compared to control group.

Conclusions

This study suggested that MEG3 and miR-181b may play a role in the progression of CS in diabetic patients.

Keywords

Main Subjects

Egyptian Journal of Chemistry
Volume 65, Issue 132 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part B)
December 2022
Pages 377-385

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History

  • Receive Date: 21 March 2022
  • Revise Date: 23 April 2022
  • Accept Date: 26 April 2022

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