The Effectiveness of Begonia Multangula Blume Leaf Ethanol Extract as Polymicrobial Antibiofilm on Catheters

Document Type : Original Article


1 Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232, Indonesia.

2 Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, Kalimantan Timur, 75124, Indonesia.

3 Study Program of Pharmacy, Health Institute of Mitra Bunda, Batam 29444, Riau Island, Indonesia.

4 Faculty of Health Science, Pharmacy Study Program, Univewrsitas Muhammadiyah Kudus, Jawa Tengah, 59316, Indonesia.

5 Department of Epidemiology Faculty of Public Health, Universitas Halu Oleo, Kendari, 93232, Indonesia

6 Faculty of medicine, Universitas Halu Oleo, Kendari Indonesia

7 Pharmaceutical Research and Development Laboratory of Pharmaca Tropis Pharmacy Faculty, Universitas Mulawarman, Kalimantan Timu, Samarinda, Indonesia.


The growth of catheter biofilm is associated with urinary tract nosocomial infections and causes an increase in mortality every year. Begonia Multangula Blume leaves are known have antibacterial activity but their antibiofilm activity has not been reported. This study aimed to determine the effectiveness of Begonia Multangula Blume extract in inhibiting polymicrobials in catheters. The research was conducted using the microtiter broth method. Antibiofilm activity was determined as the minimum biofilm inhibitory concentration (MBIC50), the minimum biofilm eradication concentration (MBEC50). Antibiofilm mechanism elucidated using scanning electron microscopy (SEM). Statistical analyzes were performed using ANOVA (P <0.05). Begonia Multangula blume leaf extract 1% v/v gave inhibitory activity of polymicrobial biofilm formation in the middle phase catheter of 65.23 % ± 0.01 and maturation phase of 60.44 % ± 0.01 almost equivalent to the control drugs chloramphenicol and nystatin 1 % v/v. The results provide evidence that the leaves of Begonia Multangula blume can eradicate 60% polymicrobial biofilms 63.55 % ± 0.01. Observations using SEM showed disruption the Extracellular Polymeric Substances (EPS) layer due to the test compound. The conclusion of this study, that Begonia Multangula blume leaf ethanol extract has the potential to be developed as a candidate for new antibiofilm drugs against polymicrobial biofilms on catheters


Main Subjects

Volume 65, Issue 132 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part B)
December 2022
Pages 195-201
  • Receive Date: 28 January 2022
  • Revise Date: 30 March 2022
  • Accept Date: 06 April 2022