Comparative proteomics analyses of female Culex pipiens mosquito gut proteins after sugar and blood feeding using Nano LC-MS

Document Type : Original Article

Authors

1 Zoology and Entomology department, Faculty of Science, Al-Azhar university, Cairo, Egypt

2 Zoology and Entomology Department, Faculty of Science, Al-Azhar University, Egypt

3 Microbiology and Immunology Department, Veterinary Research Division, National Research Centre, Dokki 12622, Giza, Egypt.

4 Zoology Department, Faculty of Science, Menoufia University, Egypt

Abstract

Culex pipiens is a major vector transmitting a variety of arthropod-borne pathogens. Cx pipiens were collected then identified morphologically and by mitochondrial cytochrome oxidase I gene for accurate species confirmation. Mosquito's' gut plays an important role in infection susceptibility. A baseline proteomic dataset is needed to give insights into the physiology of blood-feeding and to understand functional proteins in mosquito behavior following a blood meal ultimately leading to establishing effective control strategies. As the changes in protein expression of Cx. pipiens gut after sugar and blood-feeding has not been investigated, a proteomic analysis of gut tissue was carried out using Nano LC/mass spectrometry (LC-MS) and offline matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-MS. There were 56 functional putative proteins in the gut of sugar-fed Cx. pipiens females and 37 proteins gut of blood-fed Cx. pipiens females with molecular weight ranging from 800.4 to 2969.4 Da and from 818.3 to 3207.6 Da for sugar and blood-fed gut females, respectively. 39 and 20 proteins were uniquely identified in sugar-fed and blood-fed, respectively, while 17 proteins were common by the two biological groups. Data are available via ProteomeXchange with identifier PXD030533.

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History

  • Receive Date: 11 March 2022
  • Revise Date: 31 March 2022
  • Accept Date: 06 April 2022

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