Jasonia montana Extracts Stimulate Cell Differentiation in HCT-116 and Caco-2 Cell Lines

Aidy, Esraa A.; Fathy, Zeinab; Ahmed, Wafaa Abdullah; El-Gendy, Saad; Abou-Bakr, Amany; Omran, Mervat; Al-Shafie, Tamer A.

doi:10.21608/ejchem.2022.113827.5252

Jasonia montana Extracts Stimulate Cell Differentiation in HCT-116 and Caco-2 Cell Lines

Document Type : Original Article

Authors

¹ Biochemistry Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt

² Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt

³ Cancer Biology Department, National Cancer Institute, Cairo University, Egypt

⁴ Pathology Department, National Cancer Institute, Cairo University, Egypt

⁵ Department of Cancer Biology, Pharmacology Unit, National Cancer Institute, Cairo University, Cairo 11796, Egypt

⁶ Department of Biochemistry, Faculty of Dentistry, Pharos University in Alexandria

10.21608/ejchem.2022.113827.5252

Abstract

Unlike differentiated cells, cancer stem cells (CSCs) can generate malignancy. Conventional chemotherapy results in CSCs spreading causing metastasis and relapse. Differentiation is an emerging approach providing a safer strategy to eradicate CSCs. Jasonia montana is a medicinal plant that occurs in Sinai Peninsula in Egypt. Here, we used two human colorectal cancer cell lines; HCT-116 and Caco-2 to investigate the ability of methanolic and petroleum ether extracts of Jasonia montana to differentiate colon CSCs. Tandem Mass–Spectrometry (MS/MS) revealed that quercitrin and 5-Hydroxycostic acid represent the major extracts ingredients. Cytotoxicity assay (SRB) illustrated that petroleum ether extract inhibited Caco-2 cells (IC50=10 μg/ml), whereas, methanolic extract inhibited HCT-116 cells (IC50=20 μg/ml). On treating HCT-116 and Caco-2 cells with methanolic and petroleum ether extract respectively in diluted doses to alleviate their cytotoxicity, qRT-PCR showed a significant increase in CK20 expression and a significant decrease in CD44 expression, a significant decrease in Sox-2 and NOTCH-1 expression and a significant increase in PTEN expression. Furthermore, qRT–PCR showed a significant decrease in miR-21-5P and miR-10b-5P expression and significant increase in miR-34a-5P and miR-145 expression. Our results suggest, for the first time, that Jasonia montana methanolic and petroleum ether extracts can induce colorectal CSCs differentiation.

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