In Vitro antioxidant and cytotoxic evaluation of ethyl acetate fraction of Angiopteris ferox Copel tuber against HTB lung cancer cell

Aisyah, Andi Nur; Lukitaningsih, Endang; Rumiyati, Rumiyati; Marwati, Marwati; Sapra, Amriani; Khairi, Nur; Fadri, Alfat; Nur, Syamsu

doi:10.21608/ejchem.2022.91843.4364

In Vitro antioxidant and cytotoxic evaluation of ethyl acetate fraction of Angiopteris ferox Copel tuber against HTB lung cancer cell

Document Type : Original Article

Authors

¹ Department of Pharmaceutical, Sekolah Tinggi Ilmu Farmasi Makassar

² Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Gadjah Mada, Yogyakarta, Indonesia

³ Department of Pharmaceutical Biology

⁴ Department of Pharmaceutical Technology, Sekolah Tinggi Ilmu Farmasi Makassar, Indonesia 90242

⁵ Department of Pharmaceutical Chemsitry, Sekolah Tinggi Ilmu Farmasi Makassar

⁶ Department of Pharmaceutical Chemistry, Sekolah Tinggi Ilmu Farmasi Makassar

10.21608/ejchem.2022.91843.4364

Abstract

The tubers of Angiopteris ferox Copel from the Mirrataceae family have been widely used empirically by society for various treatments. This experimentation aimed to discover the antioxidant and anticancer action of the ethyl acetate (EA) fraction of Angiopteris ferox Copel tubers against HTB lung cancer and normal cells in vitro. The EA-fraction obtained from the liquid-liquid extraction was tested for antioxidant activity using the beta carotene degradation and the hydroxyl radical method. The EA-fraction tries the cytotoxic sequel on lung cancer and normal cells using the MTT test method. The outcome achieved that the EA-fraction of the sample has antioxidant activity in reducing lipid peroxidation radicals with an IC50 grade of 30.29 µg /mL (extreme category) and hydroxyl radicals with an IC50 grade of 126.62 µg /mL (medium category). In cytotoxic activity has a medium toxic effect on pulmonary HTB cell line with an IC50 grade of 78.96 µg /mL.
In comparison, normal Vero cells have a feeble harmful impact with an IC50 rate >500 µg /mL. It could have culminated that the EA-fraction of Angiopterix ferox Copel has antiradical activity and has a toxic effect on HTB cancer cells but is weak against normal Vero cells. It shall promote as a remedy for the management of lung cancer.

Keywords