Document Type : Original Article
Authors
1
Genetics and Cytology Department. Biotechnology Research Institute. National Research Centre
2
Genetics and Cytology, NRC, Cairo, Egypt.Genetics and Cytology Department, Biotechnology Research Institute , National Research Centre, 33 El Buhouth ST., 12622, Dokki, Giza, Egypt
3
Genetics and Cytology Department, Biotechnology Research Institute , National Research Centre, 33 El Buhouth ST., 12622, Dokki, Giza, Egypt
4
Genetics and cytology-NRC- Cairo-Egypt
Abstract
Two daffodil genotypes were used in a comparative study; Narcissus tazetta var. Chinensis and Narcissus tazetta cv. Wave. MS medium with different additives were used: MS free as control, MS with2,4-D for callus induction (indirect propagation), and MS with TIBA + IBA and activated charcoal for direct propagation, twin scale was used for explant inoculation, different sub-culturing times and acclimatization were applied. Four primers were used for RAPD-PCR, beside cytological examination for native bulbs of the two daffodils.
Experiments revealed that the regeneration of Narcissus showed less than moderate genetic stability to the number of subcultures, media content and through acclimatization, generally N. t. cv. Wave had more growth vigor than N. t. var. Chinensis. All measured characters revealed that MS medium with 2mg/L 2,4-D provide the chance for more callus induction, whereas MS with 1mg/L IBA + 1mg/L TIBA in addition to 1gm/L of activated charcoal gave more opportunity for explants to be vigor in leaves and roots number as well as length. The profile of RAPD-PCR analysis revealed some changes with differ in medium additives, subculture and acclimatization, reflecting that the two Narcissus genotypes have less than moderate genetic variations under three media conditions and conclude that the two genotypes of Narcissus have genetic stability under studied conditions. The two genotypes had different chromosome number demonstrated with cytological examination.
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