Nanocapsulation of curcumin and its protective effects against oxidative stress and carcinoma HepG2, MCF7 cells

Document Type : Original Article


1 Department of Agricultural Chemistry, Faculty of Agriculture , Minia University, El-Minya 61519, Egypt

2 Agric Chemistry, Minia University

3 Food Technology Dep., National Research Centre, Dokki, Egypt

4 Tumor Center, Cairo University


Curcumin, the highly active compound has restricted in the medical and nutritional applications due to its poor bioavailability and solubility. Using Ball milling technique for grinding curcuma powder at 30, 90 min and capsulated the natural extracts in micro and nano forms. Chemical components were compared between control (0 min) powder with 90 min grinded time. Comparing the non-capsulated (CM), Microencapsulated (CMm) and nanoencapsulated (CMn) as biological and chemical agents. Using HPLC-MS, it was found three main components; curcumin, curcumin glucuronid and curcumin sulfate at different ratios. Moreover, SEM microscope showed average particle size more than 450-550 µm for micro CMm 90 capsules, while TEM microscope showed spherical micelles ranged between 7 – 42 nm for nano CMn 90 capsules. Microencapsulated (CMm) and nanoencapsulated (CMn) were investigated as antioxidant and anticancer agents. Samples (66.6 µg/ml) were showed obvious DPPH scavenging activity for micro- then nanoencapsuled, and that was the same in slightly trend for Reducing power compared with non-encapsulated and ascorbic acid. Against human hepato cancer HepG2 cells, nanoencapsules were more active than micro capsules followed by non-capsuled samples in a bright selective anticancer activity. Against human breast cancer MCF7, the anticancer potential has higher IC50 (µg/ml) but in the same activity trend.


Main Subjects