Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction

Astuti, Ayun Dwi; Yasir, Budiman; Rahim, Abdul; Natzir, Rosdiana; Subehan, Subehan; Goto, Kyoko Nakagawa; Alam, Gemini

doi:10.21608/ejchem.2021.80171.3972

Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction

Document Type : Original Article

Authors

¹ Department of Medical Science, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia

² Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia

³ Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar 90245, Indonesia

⁴ Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia

⁵ School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa 920-1192, Japan.

⁶ Faculty of Pharmacy, Hasanuddin University Indonesia

10.21608/ejchem.2021.80171.3972

Abstract

Plectranthus scutellarioides is one of medicinal plants in Indonesia, traditionally known as iler/miana/jawer kotok. Due to a large number of varieties, certain varieties of P. scutellarioides have not been extensively studied. This study focused on the isolations of two phytosterols, stigmasterol and β-sitosterol, which were characterized by various 1D and 2D NMR analyses, from the ethyl acetate extract of P. scutellarioides var. color blaze dark star. Cytotoxicity of the ethyl acetate extract and some selected fractions were evaluated against A549, MDA-MB-231, MCF-7, KB, and KB-Vin cell lines using SRB (Sulforhodamine B) method. Among all tested sample, fractions 15 and 17 have significantly inhibited cell growth with IC50 of 14.3-24.3 µg/mL and 7.3-14.1 µg/mL, respectively.

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