Document Type : Original Article
Authors
1
Biochemistry, Faculty of Science, Cairo Univ
2
Professor of Biochemistry department. Faculty of Science Cairo Univ.
3
Dean of faculty of medicine MTI University.
4
Prof of clinical physiology Benha college of medicine Randa.mostafa@fmed.bu. Edu. Eg
5
Professor of Clinical pharmacology department. Ain shams University faculty of medicine.
6
Professor of anatomy and embryology Faculty of medicine ain shams university and AFCM armed force Collage of medicine.
7
Associate Professor Biochemistry Department, Faculty of Science, Cairo University, Egypt.
8
Associate professor of Biochemistry department Ain shams faculty of medicine
Abstract
Abstract
It is elevated proves that the polyphenolic compounds of natural origin, such as anthocyanins, being cyanidin-3-O-glucoside (Cy3G) one of the most widely distributed anthocyanins, may control ischemia/reperfusion (I/R) injury although how much sheltering occurs is not obvious. In the present study, the role of cyanidin-3-O-glucoside pretreatment of cardiac ischemia and dysfunction was studied. Cyanidin-3-O-glucoside in 3 doses (5, 10, and 15 mg/kg body weight), was introduced in vivo 5 min before a 45 min closure of the left anterior descending artery, followed by a 120 min reperfusion in male Wistar rats. Blood samples and cardiac tissue specimens were obtained for studying the cardiac markers and the expression of lncRNA-CTC-448F2.4, miR1273a, and mRNA-DCN (Decorin), Specimens of left ventricles were obtained and prepared for histopathological criteria. Specimens of pretreated rats showed minimal cardiac edema, hemorrhage, cellular inflammatory infiltration, and fibrosis in cardiac tissue samples. Notably, this protective administration of cyanidin-3-O-glucoside also produced a dose-dependent downregulation in lncRNA- CTC-448F2.4 and mRNA-DCN (Decorin, an important gene involved in the regulation of autophagy) with elevation in miR-1273a expressions. Cyanidin-3-O-glucoside pretreatment leads to improvements in cardiac function tests, enzymes, and myocytes. In conclusion, Cyanidin-3-O-glucoside is promising for the protection of the heart from injury following ischemia-reperfusion in the rat examination.
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