Kinetic and inhibition effect studies of ecofriendly synthesized silver nanoparticles on lactate dehydrogenase and ferritin activity

Document Type : Original Article

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Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq

Abstract

Abstract
Silver nanoparticles (AgNPs) have been one of the most attractive nanomaterials in biomedicine due to their unique physicochemical properties. In this paper, we synthesize silver nanoparticles using simple, cheap and environmentally green methods. This stage is more suited to large-scale manufacturing since it is speedy and removes the complex steps in other bio based methods (by using fungi and bacteria). The synthesis method of AgNPs include biological route using Myrtus communis leaves extract as reducing agent and silver nitrate as precursors. The prepared AgNPs were characterized using scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR) and UV-visible spectroscopy (UV) analyses. The results showed that the Myrtus communis leaves extract gave pure Ag nanoparticles with the crystallite size of 50 nm. The inhibitory effects of biosynthesized AgNPs on ferritin and lactate dehydrogenase activity in sera of covid-19 patients and the sera of healthy control subjects were investigated. The Results revealed that the inhibition percentage of AgNPs with LDH was 78.8% and 66.3 % for patient and control group respectively. While for ferritin activity, it was 87.87% for the patient group and 63.5% for the control group. Oral consent was obtained from the most patients and healthy subjects before them being under study. Besides, Kinetic studies of ferritin and lactate dehydrogenase were performed. Finally, AgNPs showed an effective biological activity toward Staphylococcus aurous and E. coli.

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History

  • Receive Date: 18 June 2021
  • Revise Date: 21 August 2021
  • Accept Date: 05 September 2021

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