The protective role of Diosmin, Hesperidine combination against heavy metals toxicity in Wistar albino rats: Biochemical, Immunohistochemical and Molecular Studies

Sarhan, Hazem Kamel; Saleh, Ahmed M.A.; Hammam, Olfat; Atta, Aly H; Elnahrery, Eslam

doi:10.21608/ejchem.2021.83002.4078

The protective role of Diosmin, Hesperidine combination against heavy metals toxicity in Wistar albino rats: Biochemical, Immunohistochemical and Molecular Studies

Document Type : Original Article

Authors

¹ Medical and Radiation department, Research sector, Nuclear Material Authority

² Head of Giza scientific office, Egyphar for Pharmaceutical Industry, El-Obbore, Cairo, Egypt

³ Theodor Bilharze institute

⁴ Chemistry Department, Faculty of Science, Suez University, Suez, Egypt

10.21608/ejchem.2021.83002.4078

Abstract

The benchmark of this study is to evaluate the antioxidant protective efficiency of Diosmin-Hesperidin combination, a natural citrus flavone of hesperidin derivative on heavy metals intoxication and Oxidative stress-induced damage in Wistar albino rats. Oral doses of diosmin-hesperidin in rats (200 and 100 mg/kg body weight, respectively) for a month (every other day) prior to heavy metals intoxication. Evaluation of the protective and antioxidant effects of the combination of diosmin and hesperidin, various Rt-PCR estimations, biochemical estimations, histopathological alterations as well as comet assay and caspase-3 activity for assessment of apoptosis were performed. Results indicated that heavy metals intoxication-induced decline in the levels of liver tissue P53 gene expression and increase of liver tissues of the apoptotic caspase-3 gene, also induced decline in the levels of liver tissue antioxidant parameters (SOD, GPx, and GSH), increased lipid peroxidation (MDA), DNA damage and apoptosis, these parameters were improved by pre-administration of diosmin+hesperidine. Diosmin+hesperidine dose (200 and100 mg/kg body wt. respectively) restored the p53 and caspase-3 genes near-normal values, antioxidant status to near normal and reduced lipid peroxidation, DNA, and tissue damage. These results were confirmed by histopathological examinations, which showed that pre-administration of diosmin+hesperidine protected the liver of albino rats against heavy metals intoxication-induced damage. Hence, it has been illustrated that diosmin+hesperidine might be an effective antioxidant and protector against heavy metals intoxication-induced damage in rats. Moreover, the diosmin+hesperidine alone pretreated group did not show any biochemical alterations, fold change of P53 or caspase-3 genes or DNA damage indicating the protective nature of the drug.

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