Comparison between immunoassay technique and a manual laboratory method for the measurement of sorbitol dehydrogenase (SDH) activity for different types of diabetes in Nineveh governorate.

Ibrahim, Maher A.; Ali, Wasan Kh.

doi:10.21608/ejchem.2021.76157.3723

Comparison between immunoassay technique and a manual laboratory method for the measurement of sorbitol dehydrogenase (SDH) activity for different types of diabetes in Nineveh governorate.

Document Type : Original Article

Authors

Maher A. Ibrahim ¹
Wasan Kh. Ali ²

¹ Department of Biochemistry, College of Medicine, University of Mosul, Mosul, Iraq

² Department of Chemistry, College of Science, University of Mosul, Iraq

10.21608/ejchem.2021.76157.3723

Abstract

In this research ,the efficacy of the sorbitol dehydrogenase enzyme was compared with immunoassay technology and laboratory method for diabetic patients in Mosul city .Types of diabetic patient were divided in to four groups whose ages range from (8-62) years of both sexes (64 males and 56 females).The first group of patients who depend on their treatment on insulin injection only known as typ1 diabetes classified, and the second group was classified for patients who depend in their treatment on pills only to treat diabetes, which is known type 2 diabetes, while the third group of gestational diabetes was classified exclusively , in the four group of patients who depend on pills and injections in their treatment, the activity of (SDH) enzyme was estimated in the serum of healthy people without any mentioned disease, and they were considered a control group. The results showed a high significant in glucose concentration in serum of patient diabetes when compared with control group, also it was observed that there was a significant increase in the activity of the (SDH) enzyme in the serum of person with diabetes in the four group when compared with the control group which measured by the immunoassay technique and by the laboratory method. the results showed a significant increase in sorbitol dehydrogenase have been compared with the control group. The relationship was a study between (SDH) activity and glucose concentration of patient and control groups by finding the linear correlation coefficient. The positive significant correlation have found between the (SDH) activity of enzyme and the glucose concentration in control and patient groups.

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