Bovine Serum Albumin Pre-concentration Using Glycidyl Methacrylate-co-ethylene Glycol Dimethacrylate-co- (2-diethylamino) ethyl Methacrylate Monolith

Document Type : Original Article

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Department of Chemistry, College of Science, University of Babylon, Iraq

Abstract

Solid Phase Extraction (SPE) is one of the important as tool in the process of Pre-concentration of some biological compounds . such as proteins based on a solid support phase that interacts with the material to be Pre-concentration and then displaces that material with a suitable solvent in the lowest volume of the solvent . monolithic sorbent was prepared using a homogeneous mixture of two monomers Glycidyl methacrylate (GMA) and (2- diethylamino) ethyl methacrylate (2DEAMA) . In the presence of a cross-linker substance Ethelyn glycol dimethacrylate (EDMA) using a suitable solvent (porogenic solvent) consisting of 1-propanol and 2-butanol and a Initiator (2,2-dimethoxy-2-phenyl acetophenone) to the polymerization proces (photoelectric) . This monolithic was prepared inside a borosilicate tube . Evaluation of monolith performance was carried out by extraction of Bovine serum albumin standard protein and the extraction recovery was in the range (89 %) .

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History

  • Receive Date: 16 March 2021
  • Accept Date: 28 April 2021

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