Indirect Spectrophotometric Determination of Mebendazole Using N-Bromosuccinimide as An Oxidant and Tartarazine Dye as Analytical Reagent

Document Type : Original Article

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Department of Chemistry, College of Education for Pure Science (Ibn Al- Haitham), University of Baghdad, Iraq

Abstract

A simple indirect spectrophotometric method for determination of mebendazol in pure and pharmaceutical formulation was presented in this study. UV-Visible spectrophotometry using the optimal conditions was developed for determination of mebendazole in pure drug and different preparation samples. The method is based on the oxidation of drug by n-bromosuccinimide with hydrochloric acid and the left amount of oxidizing agent was determined by the reaction with tartarazine and the absorbance was measured at 428 nm. Calibration curves were linear in the range of 5 to 30 µg.mL-1 with molar absorptivity 8437.2 L.mol-1 .cm-1. The limits of detection and quantification were determined and found to be 0.7770 µg.mL-1 and 2.3400 µg.mL-1 respectively. Accuracy and precision were measured by the relative error and RSD and they were found to be < 5% and < 2 respectively. Indirect analysis of mebendazole in different syrup samples was successfully applied using the proposed method.

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History

  • Receive Date: 21 March 2021
  • Revise Date: 15 April 2021
  • Accept Date: 24 April 2021

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