An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time

Maharani, Rani; Amrullah, Mufti Hanif; Kurnia, Dessy Yulyani; Hidayat, Ace Tatang; Nurlelasari, Nurlelasari; Harneti, Desi; Supratman, Unang

doi:10.21608/ejchem.2021.52601.3085

An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time

Document Type : Original Article

Authors

¹ Department of Chemistry Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia Central Laboratory Universitas Padjadjaran, Jl. Raya BandungSumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia

² Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran

³ Department of Chemsitry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran

⁴ Department of Chemistry Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia

10.21608/ejchem.2021.52601.3085

Abstract

SCAP1e is an alanine-rich-pentapeptide, analog of SCAP1 an antioxidant peptide. The previous solid-phase synthesis on this peptide resulted in low yield of the product (7%) due to the aggregation that might occur during synthesis. The aim of the research was to resynthesize this peptide using the similar method but with a slight modification in the resin loading step. SCAP1e was successfully synthesized through solid-phase method with an improved yield 87.9% and high purity (90.2%) based on the HPLC analysis. The reduction of resin loading value from 1.2 mmol/gram to 0.63 mmol/gram has made a good impact for the improvement of the synthetic yield.

Keywords