Molecular Docking, Synthesis and ADME Studies of New Pyrazoline Derivatives as Potential Anticancer Agents

Wabdan, Ammar Kadhim; Mahdi, Monther Faisal; Khan, Ayad Kareem

doi:10.21608/ejchem.2021.64042.3377

Molecular Docking, Synthesis and ADME Studies of New Pyrazoline Derivatives as Potential Anticancer Agents

Document Type : Original Article

Authors

¹ Department of pharmaceutical chemistry/ college of pharmacy/ Mustansiriya University/ Baghdad/ Iraq

² pharmaceutical chemistry department, college of pharmacy, Mustansiriyah University, Baghdad- Iraq

³ Department of Pharmaceutical Chemistry, College of Pharmacy, Mustansiriyah University, Baghdad - Iraq.

10.21608/ejchem.2021.64042.3377

Abstract

ABSTRACT
Cancer is a significant worldwide public health issue.The adverse effects of anticancer chemotherapies still compromise the quality of life of patients .To identify new potential targeted anticancer agents, a series of pyrazoline derivatives were synthesized and evaluated for anticancer effects on A549 (human lung adenocarcenoma cell line). The new derivatives were synthesized by incorporating pyrazoline pharmacophore into nabumetone moiety as a starting molecule and the chalcone derivatives as intermediate products. In silico evaluation methods were done before synthesis through .molecular docking via genetic optimization for ligand docking (GOLD) Suite software with EGFR tyrosine kinase and exhibited significant tyrosine kinase inhibition activities compared with erlotinb as a reference drug due to their hydrogen bonding interaction with key amino acids and these results are compatible with the experimental findings . The Compound structures were conﬁrmed by 1H-NMR , some physicochemical properties and infrared spectroscopy . An in vitro assay demonstrated that the final compounds (P1,P2,P3 and P4) exerted potent to moderate cytotoxic activity in the micromolar range with an IC50 values (15.409µM, 7.24µM, 27.05µM, 22.45µM) respectively when compared with erlotinib (IC50 =25.23µM) while (P5&P6) show weak activity . Pharmacokinetic and physicochemical properties of the final compounds predicted by using ADME Evaluations . The results showed all the synthesized compounds have high oral bioavailability and good GI absorption.

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