Synthetic Insecticide Fipronil Induced Over Gene Expression, DNA and Liver Damage in Female Rats: The Protective Role of Fish Oil

Refaie, Amel A.; Ramadan, Amal; Sabry, Nevien M.; Khalil, Wagdy K. B.; Mossa, Abdel-Tawab H.

doi:10.21608/ejchem.2021.58506.3264

Synthetic Insecticide Fipronil Induced Over Gene Expression, DNA and Liver Damage in Female Rats: The Protective Role of Fish Oil

Document Type : Original Article

Authors

¹ Environmental Toxicology Research Unit (ETRU), Pesticide Chemistery Department, National Research Centre, P.O. 12622 Dokki, Giza, Egypt

² Department of Biochemistry, Genetic Engineering and Biotechnology Research Division ,National Research Centre (NRC), P.O. 12622, Dokki, Giza, Egypt

³ Cell Biology Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza, Egypt

10.21608/ejchem.2021.58506.3264

Abstract

Synthetic insecticide fipronil (FPN) is widely spread worldwide for controlling insects in agriculture and public health sectors. Thus, this work aimed to study the molecular mechanisms of FPN induced hepatotoxicity and the protective role of fish oil in female rats. Female rats were allocated in four groups of rats exposed to FPN and/or fish oil and control. Oxidative stress markers i.e., lipid peroxidation (LPO), antioxidant enzymes, and liver function enzymes, butyryl cholinesterase (BuChE), histopathological lesions, DNA damage, expression alteration of apoptosis and inflammatory related genes in liver tissues were assessed. FPN treatment induced significant changes in the levels of LPO, liver function, BuChE, histopathological lesions, over-expression of tested genes and elevation in DNA damage. In contrary, treatment of FPN-exposed rats with fish oil improved significantly the negative effects of FPN on liver tissues. The molecular mechanisms of FPN might be due to the formation of reactive oxygen species (ROS) and change the oxidant/antioxidant statues. The hepatoprotective effect of fish oil might be attributed to increase the antioxidant capacity prevented the ROS generation induced by FPN in female rats.

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