Phytochemical Constituents of Ulva Lactuca and Supplementation to Improve The Nile Tilapia (Oreochromis Niloticus) Haemato-Biochemical Status

Document Type : Original Article

Authors

1 Prof.Dr. at zagazig university department of Organic chemistry

2 Central laboratory for aquaculture research, fish nutrition department, Egypt

3 Zagazig University, faculty of veterinary medicine, biochemistry department, Egypt

4 Head of department of Phytochemistry and Plant Systematics,Division of Pharmaceutical Industries,National Research Center, El Buhouth st., Dokki Dokki, Cairo, Egypt

Abstract

This study was carried out to investigate the phytochemical constituents and biological screening of green algae (Ulva lactuca ) methanolic crude extract. The HPLC spectrum profile identified seven phenolic and flavonoid compounds namely: catechin, chlorogenic ,caffeic, quercetin, and major compounds, ellagic acid with 60.87 % and rutin with 33.40% of total area. The GC profile of fatty acids portions represented to relative distribution percentage were 9 compounds, the major fatty acids are lauric acid 55.0 % , caprylic acid 21.2 % , capric Acid was 19.8 and palmitic acid was 1.94% . The 17 amino acids produced from GC spectrum , the percentage of total amino acid were 25.98% .The most abundant amino acids were glutamic acid 2.34% , alanine 2.22 and aspartic 2.09, leucine 1.33 , valine 1.26 , phenylalanine 1.0 , therionine 0.98 and isoleucine 0.9%. of total amino acid. Tilapia (Oreochromis Niloticus) diets were divided into Ulva fermentation and multi enzymes, groups each with three different supplementations (LC, PRO, and LC + PRO). The result recorded the diets containing fermented Ulva with L-carnitine, probiotic ( LC+PRO) was the highest level of red blood cell ( RBCs , HGB) by 1.97 cmm , 10.63 g/d . Similarly, increase of Hematocrit (HCt) , WBCs, platelets, and lymphocytes due to the highest level of protein, albumin, and globulin in this diets also, U. lactuca contains a number of complex carbohydrates and polysaccharides.
This study has revealed that U. lactuca seaweed is a rich source of protein ( 22% ) which is nutritionally superior to the terrestrial plant proteins and can be used to provide significant proportions of the protein requirements of fish as well as for human.

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