TY - JOUR ID - 215552 TI - Phytochemical Profiling by GC-MS Analysis and Antimicrobial Activity Potential of In vitro Derived Shoot Cultures of Some Egyptian Herbal Medicinal Plants JO - Egyptian Journal of Chemistry JA - EJCHEM LA - en SN - 0449-2285 AU - Arafa, Nermeen Mohammad AU - Girgis, Nancy AU - Ibrahem, Mona AU - Mohamed, Sahar AU - El-Bahr, Mohamed Kamal AD - Plant Biotechnology,Genetic Engineering and Biotechnology Division,National Research Center,Dokki,12622,Cairo,Egypt AD - Plant Biotechnology,National Research Center,Dokki,12622,Cairo,Egypt AD - Microbial Biotechnology,National Research Center,Dokki,12622,Cairo,Egypt AD - Plant Biotechnology, National Research Center, Dokki 12622, Cairo, Egypt Y1 - 2022 PY - 2022 VL - 65 IS - 9 SP - 155 EP - 169 KW - medicinal plants KW - multiplication KW - shootlets KW - NAA KW - BAP KW - GC-MS analysis KW - antimicrobial activity DO - 10.21608/ejchem.2022.115045.5230 N2 - The Egyptian climate possesses many herbal medicinal plants, three species namely carnation (Dianthus caryophyllus), thyme (Thymus vulgaris L) and basil (Ocimum basilicumL) were selected to study in this research. The current study was designed to optimize a protocol of in vitro propagation for progeny uniformity mass production of the selected species to avoid their genetic and biochemical heterogeneity. Nodal segments of carnation, thyme and basil seedlings cultured on medium contained benzyl amino purine (BAP) at different concentrations with or without naphthalene acetic acid (NAA) at 0.5 mg/l. The presence of NAA combined with a high level of BAP was more efficient for shootlets mass production of all studied plant species. Shootlets were extracted using diethyl ether, most of their substantial phytocomponents (fatty acid derivatives, essential oils, , cyclohexane carboxylic acid, hydrocarbons, etc.,) were detected in different proportions by GC-MS analysis. Shootlets extracts were evaluated antimicrobial in vitro, and exhibited differentially good performance against Bacillus subtilis, Staphylococcus aureus, Pseudomonas floureceans, Escherichia coli and Candida albicans. Diameters of inhibition zones ranged from 16.133±0.696 to 23.466±0.837mm for all extracts. In conclusion, in vitro normal healthy shootlets of carnation, thyme and basil were obtained, and their extracts were rich in bioactive phytochemicals which in turns play a potent role for microbial growth inhibition. UR - https://ejchem.journals.ekb.eg/article_215552.html L1 - https://ejchem.journals.ekb.eg/article_215552_536d2bf32a6b206a29d8edd6490b9f27.pdf ER -